Calibration & Standard Curve Management¶
Calibration is what turns a raw fluorescence signal into a meaningful concentration. This page explains how the FIA680/FIA880 manage standard curves — and why the operator never builds one manually.
Why Pre-Loaded Curves Matter¶
A standard curve maps fluorescence intensity to analyte concentration. Manually constructing curves is the single largest source of inter-operator variability in quantitative testing. Migibio removes this by pre-loading validated curves.
| Curve method | How it works | Operator effort |
|---|---|---|
| QR code (FIA680) | Scan code → curve loads | Scan only |
| Cloud sync (FIA880) | Curve pushed automatically | None |
| Manual construction | Operator builds curve | High (error-prone) |
The Calibration Chain¶
Each production lot is validated against a standard curve during manufacturing; that curve is then delivered to the analyzer via QR code or cloud sync.
QC Verification¶
Even with pre-loaded curves, routine QC confirms the system is reading correctly:
| QC step | Frequency | Purpose |
|---|---|---|
| QC cartridge | Daily | Verify curve integrity |
| Lot-change verification | Per new lot | Confirm new curve loads |
| Full calibration | Per service schedule | Instrument-level check |
What This Means for Results¶
- No manual curve errors — the operator cannot mis-enter a curve.
- Lot-specific accuracy — each lot uses its own validated curve.
- Consistency across operators — two operators get the same result from the same sample.
FAQ¶
Do I need to recalibrate between lots? When you receive a new assay lot, load the new lot's curve (QR scan or cloud). No manual recalibration is required.
How do I know the curve is valid? Daily QC cartridge verification confirms the analyzer reads the expected control value.
For maintenance schedules, see the FIA680 and FIA880 guides.
Authored by: Migibio Clinical & Scientific Affairs, Guangzhou Magic Biotech Co., Ltd.
Reviewed by: Migibio R&D Quality Committee
Last updated: 2026-08-13
Disclosure: Migibio (Guangzhou Magic Biotech Co., Ltd.) is the manufacturer of the FIA680/FIA880 analyzers and FICT reagents referenced in this content. See our Editorial & Review Policy.